Restriction and modification enzymes. PCR. DNA sequencing. Hybridization of DNA. Vectors and plasmids. Reporter genes. Libraries. Heterologous protein production. Methods for genes expression analysis, metylation analysis and study of protein DNA interaction. Methods for the study of genetic polymorphism. Techniques for GMO. Mutagenesis. CRISPR. Synthetic biology
Papers and slides suggested during classes.
Texbooks of genetic engineering:
Primrose, Twiman & Bold, Principles of Gene Manipulation
Brown, Gene Cloning and DNA Analysis: An Introduction
Learning Objectives
Knowledge acquired: Basic and advanced knowledge genetic engeneering. Knowledge on methodologies for genetic maniplation of organisms
Competence acquired
The student has acquired competence on the methods for DNA analysis and manipulation
Skills acquired (at the end of the course):
The student is able to autonomously set up an experimental and protocol for DNA analysis.
Prerequisites
Recommended classes on Genetics and Molecular Biology
Teaching Methods
Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other individual formative activities: 102
Contact hours for: Lectures (hours): 48
Contact hours for: Laboratory (hours):
Contact hours for: Laboratory-field/practice (hours): 0
Seminars (hours): 0
Stages: 0
Intermediate examinations: 0
Further information
Office hours:
Upon request from Monday to Friday
Type of Assessment
Exam modality: written
Duration: 1h
Type of questions: open and closed questions
Aim: verifiy knowledge and competences (problem solving)
Course program
Restriction and modification enzymes. PCR. DNA sequencing. Hybridization of DNA. Vectors and plasmids. Reporter genes. Libraries. Heterologous protein production. Methods for genes expression analysis, metylation analysis and study of protein DNA interaction. Methods for the study of genetic polymorphism. Techniques for GMO. Mutagenesis. CRISPR. Synthetic biology