Basic concepts in Magnetic Resonance. Laboratory of NMR Spectroscopy: hands on, practicals and supervised works. Applications of Magnetic Resonance in Structural Biology, metabolomics, diagnostics, drug screening and drug desing, protein-protein interactions.
Isotope labeling techniques and protein preparation.
P.J Hore, Nuclear Magnetic Resonance, Oxford Chemistry Press
-P.J. Hore, J.A Jones, S. Wimperis, NMR: the toolkit, Oxford Chemistry Press
-M H Levitt, Spin dynamics, Wiley.
Research and Review articles to be distributed during the class
Learning Objectives
The course is aimed to provide the students with knowledge and understanding of Magnetic Resonance basic principles, in order to allow students to analyze possibilities and limitations of magnetic resonance in terms for spectroscopic, structural, analytic and diagnostic applications.
The applications presented throughout the course allow the students to make their own judgements on the state of the art in the application and perspectives of Magnetic Resonance in the various fields, with the aim to enable students to evaluate the possible application of MR in their own field of interest/research and to apply their acquired knowledges to design their own scenarios where MR could have a strategic role.
Prerequisites
Required courses: Elementi di matematica e Statistica,Fisica, Chimica generale e Inorganica, Chimica organica, Biologia Generale
Teaching Methods
In-front teachings, powerpoint presentations. Supervised laboratory work. Discussion groups
Type of Assessment
Dates of examinations are available at: https://sol.unifi.it/docprenot/docprenot
6-8 sessions per year. Examination will consist in a oral colloquium of about 20-30 minutes.
Topics and Aims of the oral colloquium: -Assessment of the acquired base knowledge of magnetic resonance principles; -Discussion on possible application to different aspects of contemporary biology.
Course program
Magnetic Resonance principles: NMR active isotopes, chemical shift, scalar and dipolar coupling, relaxation rates. 1D amd multi-D experiments, analysis and inteprettion of different NMR spectra.
Applications: structural biology, protein dynamics, protein-protein interactions, drug screening and drug design, metabolomics, imaging and diagnostics