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B021437 - DIAGNOSTIC MICROBIOLOGY
Main information
Teaching Language
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Further information
Type of Assessment
Course program
Academic Year 2016-17
Course year
First year - Annualità singola
Belonging Department
Biomedical, Experimental and Clinical Sciences "Mario Serio"
Course Type
Single education field course
Scientific Area
-
Credits
2
Teaching Hours
16
Teaching Term
03/10/2016 ⇒ 30/04/2018
Attendance required
No
Type of Evaluation
Giudizio Finale
Course Content
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Course program
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Lectureship
Teaching Language
Italian
Course Content
Analysis of the main molecular and traditional techniques to search for bacteria, fungi and viruses: techniques for the growth and phenotypic identification; molecular and innovative rapid techniques
Suggested readings (Search our library's catalogue)
material provided by the teachers
Learning Objectives
Knowledges of the main molecular techniques and traditional search for infectious agents
Prerequisites
None
Teaching Methods
Lectures in the presence and in the laboratory
Further information
None
Type of Assessment
Written examination with a multiple choice test
Course program
Part I: diagnostic techniques
1- Methods of isolation and titration of the virus; Evaluation of viral growth in vitro; identification and typing of virus isolates
2- Search of antigens in microbiological diagnostics
3- Search of nucleic acids in microbiological diagnostics, with particular interest to the choice of the target in relation to the different diagnostic objectives. Extraction of DNA and RNA, techniques of hybridization, amplification techniques (with particular reference to the use of PCR, RT-PCR and quantitative PCR in Virology) of the target, the signal and of the probe.
4- The analysis of the sequences in microbiology: from RFLP sequencing, to microarrays for genotyping (Main applications). Next generation sequencing and other innovative methods emerging.
5- Serological techniques in microbiology and use of recombinant antigens.
Part II: Special problems in the diagnosis of infection.
1- the diagnosis of infection in immunodepressed patients, in particular in transplantation.
2- infections and autoimmune diseases
1- Methods of isolation and titration of the virus; Evaluation of viral growth in vitro; identification and typing of virus isolates
2- Search of antigens in microbiological diagnostics
3- Search of nucleic acids in microbiological diagnostics, with particular interest to the choice of the target in relation to the different diagnostic objectives. Extraction of DNA and RNA, techniques of hybridization, amplification techniques (with particular reference to the use of PCR, RT-PCR and quantitative PCR in Virology) of the target, the signal and of the probe.
4- The analysis of the sequences in microbiology: from RFLP sequencing, to microarrays for genotyping (Main applications). Next generation sequencing and other innovative methods emerging.
5- Serological techniques in microbiology and use of recombinant antigens.
Part II: Special problems in the diagnosis of infection.
1- the diagnosis of infection in immunodepressed patients, in particular in transplantation.
2- infections and autoimmune diseases