Analysis of the main techniques for the detection of bacteria, fungi and viruses: techniques for the growth and phenotypic identification of these infectious agents; molecular and innovative rapid techniques
As educational material will be provided by the teachers of the slides of the lectures and protocols of the techniques applied during laboratory exercises
Learning Objectives
Knowledge and understanding: Main technical standards and innovative for the cultivation, identification and typing of microorganisms and viruses. Susceptibility testing to antibiotics and antivirals.
Applying knowledge and understanding: Ability to apply the appropriate techniques both traditional and molecular identification of microorganisms and viruses mainly involved in human infections
Prerequisites
Laboratory safety course and medical examination
Teaching Methods
Theoretical / practical Lessons and laboratory
Further information
None
Type of Assessment
Verification test written with multiple choice test
Course program
Topic 1: Microbiology Laboratory: Tools and procedures for working safely in the laboratory
Topic 2: Bacteriology:
1- culture media (standard, enhanced, selective and differential) and procedures for bacterial isolation: fresh examination, isolation and culture of bacteria in soil solids and liquids; macroscopic examination bacterial colonies; Gram stain; Ziehl-Neelsen.
2- Techniques for typing bacterial biochemistry (rapid and automatic)
3- Techniques for sensitivity to antibiotics: determination of MIC (Method of dilutions in broth; Method of dilutions in agar; Kirby Bauer test (agar diffusion); automated systems.
4- Molecular biology techniques applied to bacteriology
5- Using bacteria in biotechnology
Topic 3: Mycology:
1 Illustration of the main methods of cultivation and land used for fungi
2-box colors identifying and biochemical tests for identification of yeasts
3-Using yeast in biotechnology
4-molecular techniques in the identification of fungi
Topic 4: Virology:
1-Using cell cultures for the isolation of animal viruses
2-Observation of cytopathic effects and viral inclusion bodies
3-physical and biological viral titration (method of plaques and TCID50)
4-Techniques to search for viral antigens (ELISA, WB, IFA)
5-Technical hemagglutination, hemagglutination inhibition, neutralization
6-Techniques for analysis of viral susceptibility to antivirals: phenotypic and genetic
7-Use of animal viruses and not in biotechnology
8- Bacteriophages and method of cultivation: application examples in bacterial typing
9-Molecular biology techniques in the identification and quantification of viral
10-Use of computer programs and databases for the study of genetic viral
Topic 5: Serology:
1-Main serological techniques for research antibodies specific for viral and bacterial infections