Genetics General: Mendelian principles; Linkage and genetic maps; Mutations and mobile genetic elements
Genetic Breeding: Quantitative traits; Systems of reproduction; Origin and evolution of crops; Genetic breeding of autogamous and allogamous species
Genomics and Biotechnologies: In vitro cultures and biotechnologies; Molecular markers and genomic analysis; Analysis of gene expression and bioinformatics; Genomic characterization, gene mapping and assisted selection
Genetica e Genomica-Barcaccia e Falcinelli- 2008 Liguori Editore-ISBN-13 978-88-207-3743-6
Biologia cellulare & biotecnologie vegetali di Gabriella Pasqua-2011 Editore: Piccin-Nuova Libraria-ISBN: 8829921246
Learning Objectives
Knowledge and skills: acquiring knowledge of genetics applied to plants, understanding the basics of new methods of genetic analysis made available by genomics and the tools of recombinant DNA
Prerequisites
Basic knowledge in Plant Biology, Biochemistry and Genetics are required
Teaching Methods
Total hours of the course: 150 (=6 x 25)
Hours reserved to private study and other indivual formative activities: 102
Contact hours for: Lectures (hours): 32
Contact hours for: Laboratory-field/practice (hours): 16
Further information
Attendance to lectures and other activities, although not mandatory, is strongly recommended
Type of Assessment
Written test with multiple-choice questions on all course’s topics.
Questions are formulated to verify that the student, in addition to the acquisition of specific knowledge, achieved the ability to elaborate and correlate the topics examined during the course.
Course program
Genetics
-The plant cell and its genomes: Plant cell; Nuclear genome; Genomes of cytoplasmic organelles; Regulation of gene expression; Epigenetics and Epigenomics
- Nuclear genome analysis of plants: structure, expression and functions; DNA sequencing: classical methology and NGS; Genome sequencing strategies: hierarchical method and shotgun; Plant genome sequencing projects; Comparative genomics and synteny; Analysis of genetic variability: from morphological markers to molecular markers; Analysis of single gene expression and of the transcriptome; Analysis of single protein expression and of the proteome; Metabolomics; Use of mutants for the gene function study
Genetic breeding
- Principles of inferential statistics;
- Quantitative characters;
- Reproductive systems: Reproductive systems in plants; Developmental biology; MADS box; Incompatibility; Apomixes
- Population genetic structure: Population genetic structure and population dynamics; Hardy-Weinberg law; Genetic variability in natural populations
- Origin and evolution of crop species: Origin of crop species; Phylogenesis of the main crop species; Domestication; Role of mutations; Comparative genomics; Conservation of genetic resources
- Genetic breeding of autogamous and allogamous species - Objectives of genetic breeding; Genetic breeding strategies
Genomics and Biotechnology
- In vitro cultures and biotechnologies;
-Elements of genetic engineering: transgenic varieties
-Genomic characterization, genetic mapping and assisted selection