Principles of microscopic technique
Cell membrane, cytoplasm, nucleus; karyocinesis, apoptosis.
Epithelia, connective tissue, cartilage, bone, muscle tissue, nervous tissue; blood, haematopoiesis;
Fertilization and early phases of human development; implantation; formation of tissues and of the body plan of the human embryo; derivatives of the germ layers and of mesenchyme; adnexa of the embryo and foetus; foetal circulation and its modifications at birth.
Course Content - Part B
Principles of microscopic technique
Cell membrane, cytoplasm, nucleus; karyocinesis, apoptosis.
Epithelia, connective tissue, cartilage, bone, muscle tissue, nervous tissue; blood, haematopoiesis;
Fertilization and early phases of human development; implantation; formation of tissues and of the body plan of the human embryo; derivatives of the germ layers and of mesenchyme; adnexa of the embryo and foetus; foetal circulation and its modifications at birth.
Bani e collaboratori, Istologia Umana, Idelson-Gnocchi Editore. ISBN 978-88-7947-541-9
Amato e collaboratori, Embriologia Umana, Idelson-Gnocchi Editore. ISBN: 978-88-7947-539-6
Learning Objectives - Part A
Knowledge:
• principles of microscopic technique;
• cells, extracellular matrix, and tissues of the human body;
• steps and regulation of cell proliferation, differentiation, and human development.
Know-how:
• recognize normal cells and tissues of the human body;
• recognize chorionic villi and the respective phase of pregnancy;
• recognize the main cellular and extracellular structures in electron photomicrographs.
Learning Objectives - Part B
Knowledge:
• principles of microscopic technique;
• cells, extracellular matrix, and tissues of the human body;
• steps and regulation of cell proliferation, differentiation, and human development.
Know-how:
• recognize normal cells and tissues of the human body;
• recognize chorionic villi and the respective phase of pregnancy;
• recognize the main cellular and extracellular structures in electron photomicrographs.
Prerequisites - Part A
Basic knowledge of chemistry, physics and biology according to high school programs
Prerequisites - Part B
Basic knowledge of chemistry, physics and biology according to high school programs
Teaching Methods - Part A
Lectures (including projections of slides of light and electron microscopy) and e-learning through on line distributed slides and videos
Teaching Methods - Part B
Lectures (including projections of slides of light and electron microscopy) and e-learning through on line distributed slides and videos
Type of Assessment - Part A
Oral examination and analysis and identification of cells and tissues in light microscopic slides and electron photomicrographs. Admission to the oral examination is achieved by answering correctly at least 80% questions of a multiple choice test.
Type of Assessment - Part B
Oral examination and analysis and identification of cells and tissues in light microscopic slides and electron photomicrographs. Admission to the oral examination is achieved by answering correctly at least 80% questions of a multiple choice test.
Course program - Part A
Instruments and methods for the microscopical analysis of cells and tissues, principles of histochemical methods.
General features of cells: cell membrane, organelles, hyaloplasm, inclusions; specializations of the cell surface; structure of nucleus, karyokinesis, chromosomes; mechanisms for the homeostasis of cell populations, including apoptosis.
Epithelial tissues: simple (including endothelium), pseudostratified and stratified lining epithelia; exocrine glandular epithelia; endocrine glandular epithelia, non-epithelial endocrine tissues; general features of neuroepithelia.
Connective tissues: extracellular matrix of connective tissues, including basement membrane; autochthonous and blood-derived cells of connective tissues; connective tissues proper; cartilage (all types); bone tissue; osteogenesis.
Blood: red and white blood cells and their life cycle, platelets; myeloid and lymphoid haematopoiesis, features of microscopically identifiable steps.
Muscle tissues: smooth muscle tissue; striated muscle, skeletal and cardiac.
Nervous tissue: neuron; glia; nerve fibers; synapsis; peripheral nerve endings.
Embryology: ovary and menstrual cycles, gametes; elementary morphogenetic processes; general features of stem cells, cell differentiation; principal control mechanisms of embryo development; first week of human embryo development, from zygotes to blastocysts; implantation; evolution of embryoblast in the second week from conception; third week of human embryo development (formation of primitive germ layers and notochord, organization of body scheme); neural plate, neural tube, neural crest, formtion and evolution of encephalic vesicles, of the layers and laminae of neural tube and of neural crest; formation and development of surface ectoderm and its derivatives, including stomodeum, proctodeum and pharyngeal arches and grooves ectoderm, placodes and their destiny; differentiation of endoderm to form primitive intestine, differentiation of pharyngeal endoderm (including laryngotracheal bud and its derivatives), of trunk intestine and cloaca; differentiation of paraxial mesoderm into primordia of skeletal and muscle systems, differentiation of intermediate mesoderm into primordia of kidneys and urinary tract, differentiation of lateral plate mesoderm into coelomatic epithelium and its derivatives; origin and differentiation of germ cells in both sexes; formation and differentiation of mesenchyme into blood islets, angioblasts and primordia of blood vessels and heart; prenatal life haematopoiesis; formation and evolution of primitive cardiac chambers; primitive vein systems, primitive aortae, aortic arches and their destiny; foetal circulation and circulatory modifications at birth; differentiation of embryonal and foetal adnexa (amnios, vitelline sac, allantois, chorion, umbilical cord, placent), macroscopic amd microscopic aspect of placenta and umbilical cord.
Course program - Part B
Instruments and methods for the microscopical analysis of cells and tissues, principles of histochemical methods.
General features of cells: cell membrane, organelles, hyaloplasm, inclusions; specializations of the cell surface; structure of nucleus, karyokinesis, chromosomes; mechanisms for the homeostasis of cell populations, including apoptosis.
Epithelial tissues: simple (including endothelium), pseudostratified and stratified lining epithelia; exocrine glandular epithelia; endocrine glandular epithelia, non-epithelial endocrine tissues; general features of neuroepithelia.
Connective tissues: extracellular matrix of connective tissues, including basement membrane; autochthonous and blood-derived cells of connective tissues; connective tissues proper; cartilage (all types); bone tissue; osteogenesis.
Blood: red and white blood cells and their life cycle, platelets; myeloid and lymphoid haematopoiesis, features of microscopically identifiable steps.
Muscle tissues: smooth muscle tissue; striated muscle, skeletal and cardiac.
Nervous tissue: neuron; glia; nerve fibers; synapsis; peripheral nerve endings.
Embryology: ovary and menstrual cycles, gametes; elementary morphogenetic processes; general features of stem cells, cell differentiation; principal control mechanisms of embryo development; first week of human embryo development, from zygotes to blastocysts; implantation; evolution of embryoblast in the second week from conception; third week of human embryo development (formation of primitive germ layers and notochord, organization of body scheme); neural plate, neural tube, neural crest, formtion and evolution of encephalic vesicles, of the layers and laminae of neural tube and of neural crest; formation and development of surface ectoderm and its derivatives, including stomodeum, proctodeum and pharyngeal arches and grooves ectoderm, placodes and their destiny; differentiation of endoderm to form primitive intestine, differentiation of pharyngeal endoderm (including laryngotracheal bud and its derivatives), of trunk intestine and cloaca; differentiation of paraxial mesoderm into primordia of skeletal and muscle systems, differentiation of intermediate mesoderm into primordia of kidneys and urinary tract, differentiation of lateral plate mesoderm into coelomatic epithelium and its derivatives; origin and differentiation of germ cells in both sexes; formation and differentiation of mesenchyme into blood islets, angioblasts and primordia of blood vessels and heart; prenatal life haematopoiesis; formation and evolution of primitive cardiac chambers; primitive vein systems, primitive aortae, aortic arches and their destiny; foetal circulation and circulatory modifications at birth; differentiation of embryonal and foetal adnexa (amnios, vitelline sac, allantois, chorion, umbilical cord, placent), macroscopic amd microscopic aspect of placenta and umbilical cord.